Most recreational users of Psilocybe cubensis (when grown in vitro) require a dosage of 1 to 2 gm of dried mushrooms to produce an altered state of consciousness; a clinical dosage for Psilocybe cubensis, on the other hand, had previously been reported as ranging from 3 to 5 gm of dried material. This dosage would be comparable to the amount of fungal material consumed for religious purposes in a Mazatec Indian healing and curing ceremony. In 1982, one research team "found that the level of psilocybin and psilocin varies over a factor of 4 among various in vitro cultures of Psilocybe cubensis, while specimens from outdoors varied tenfold." A fresh dosage of Psilocybe cubensis in Australia would be approximately from 1 to 2 large mushrooms weighing up to as much as one fresh ounce, or as many as from 25 to 50 small mushrooms equaling the same weight amount. Ethnopharmacologist Jonathan Ott (1976, 1993) noted that he has observed "the ingestion of from 0.5 gm to 5.9 gm dried weight (10 gm to 40 gm fresh)", of various species of Psilocybe. Dosage for Psilocybe subcubensis would be the same as for Psilocybe cubensis. Both of these latter two species are macroscopically alike. The usual dosage for Copelandia cyanescens required to induce psychedelic visual effects ranges from 1 to 3 large specimens (cap diameter c. 5 mm), or as many as 5 to l0 medium-sized mushrooms (cap diameter c. 2.5 mm); however, personal tolerance to this species may occur with continued use, and some who consume large amounts of this mushroom have reportedly ingested as many as 50 to 200 fresh specimens of various sizes.The Mazatec Indians, who have a long tradition of using the mushrooms, inhabit a range of mountains called the Sierra Mazateca in the northeastern corner of the Mexican state of Oaxaca. The shamans in this essay are all natives of the town of Huautla de Jimenez. Properly speaking they are Huautecans; but since the language they speak has been called Mazatec and they have been referred to in the previous anthropological literature as Mazatecs, I have retained that name, though strictly speaking, Mazatecs are the inhabitants of the village of Mazatlan in the same mountains.
More than half of Australia's beef cattle can be found in the coastal areas of Queensland and New South Wales; and the 20 to 30 inch (500-750mm) rainfall belt of Queensland, New South Wales and Northern Victoria, generally provide adequate climatic environments for the growth of psilocybian mushrooms, especially after heavy rains. It has been suggested that "Psilocybe cubensis was introduced into Australia accidentally by early settlers along with their livestock." This same spore dispersal mechanism also probably applies to Copelandia cyanescens, Panaeolus subbalteatus and several additional species known to occur in or around the dung of other ruminants. This includes Psilocybe semilanceata and the non-hallucinogenic "haymaker's" mushroom Panaeolina foenisecii. While cattle are raised in all Australian states, as well as in the central lowlands, recreational users have been known to export these psychoptic species to various areas in Australia from areas where they were collected. In the case of New Zealand, hereafter referred to as NZ, cattle are the primary source for Copelandia cyanescens, but the "liberty cap" mushroom Psilocybe semilanceata only grows in the manured soil of four-legged ruminants and not directly from manure (Jansen, Pers. Comm., 1988). The identification section of this guide documents reported locations for more than 1 dozen species of psilocybian mushrooms in Australia and NZ which most likely have been used at one time or another for recreational purposes. It has been suggested by an Australian physician that the general public in Australia, as well as members of its drug using subculture, Most recreational users of Psilocybe cubensis (when grown in vitro) require a dosage of
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The majority of adverse physical effects or negative psychological reactions produced by "magic mushrooms" generally result from inappropriate set and expectation, or because of improper dosage, which may vary considerably among consumers, different mushroom species, or even within an individual species. The question of dosage is often confused by the variation in the source of the hallucinogenic mushroom species which is consumed. For example, Psilocybe cubensis, when picked and eaten from its natural dung (manure) habitat, produces a relatively mild mindaltering experience, which is evident from the large amounts of fresh specimens needed to achieve a threshold experience. However when grown in vitro (indoor laboratory cultivation and/or illicit cultivation), Psilocybe cubensis apparently can produce a more potent strain capable of inducing a very intense visual, sometimes quite disturbing, experience. This dosage assumes that the consumption of 1 to 3 gm of dried material would be too low if the mushroom specimen came from a wild source. This low potency for Psilocybe cubensis has been confirmed by research scientists Margot & Watling, (1981), who were surprised by the comparatively small amounts of psilocybin and psilocin which they extracted from wild specimens collected from five different locations in Australia. This suggests that a much larger dose would be required to produce significant hallucinations. It is possible that the chemicals most likely degenerated between the time that they were harvested and the time of analysis. However, it should be noted that a strain of Psilocybe cubensis producing different flushes (harvests) will vary somewhat in potency between flushes.
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This document provides complete directions for cultivating psilocybin